Safety Assessment of Glyphaea brevis Spreng. (Tiliaceae): Acute and Subacute Toxicity of the Leaf Aqueous Extract in Mice and Wistar Rats

Background: Despite the various medicinal applications of Glyphaea brevis, no toxicology data are available that could guarantee its safety or describe possible toxic effects. We studied acute and subacute toxicity leaf aqueous extract G. brevis (GbAE) in animal models.
 Materials methods: In study, mice were given a single oral administration GbAE at doses 1000, 2000 5000 mg/kg body weight (bw). The animals monitored for behavioral changes mortality over 48-hour period, thereafter 14 days. rats administered 300, 600 1200 bw daily, 28 Tissue specimens liver kidneys subjected to histological examination using standard hematoxylin-eosin staining. An array hematological (blood cells count morphology) biochemical assessments blood (ALT, AST, urea, uric acid creatinine) performed.
 Results: dose caused some signs was higher males than females. Subacute study revealed significant increase lymphocytes rate as well serum rates ALT creatinin. Microscopically, there slight hepatic renal tissue inflammation reversible.
 Conclusion: Some caution should be taken when leaves repetitively long periods. Additional preclinical toxicological acquired ascertained repeated long-term studies.
 Keywords: toxicity, histopathology, liver, kidney